PEPTIDE PROTEIN CHEMISTRY FACILITY
The Peptide and Protein Chemistry Core Facility at the University of Colorado, Anschutz Medical Campus provides state-of-the-art instrumentation for solid-phase peptide synthesis using Boc- and Fmoc-chemistry, peptide purification by reversed-phase
HPLC and peptide identification by mass spectrometry (LC-MS) services for academic and industrial clients.
Core Facility personnel will help users select and design the appropriate peptide sequences for their applications including how and
where to incorporate fluorescent and biotin labeled probes, incorporation of modified and isotopic labeled amino acids and covalent constraints to stabilize the peptide conformation, etc.
Automated Solid-Phase Peptide Synthesizers
• AAPPTec Focus XC-4RV for Boc- and Fmoc-chemistry
• CEM Liberty Microwave for Fmoc-chemistry
Analytical and Preparative HPLC Systems
• Agilent 1100 and 1200 HPLC analytical systems
• Agilent 1100 LC/Electrospray MS system
• Beckman System Gold preparative HPLC system (10 ml/min max.)
• Sharp Semi-prep Gradient Purification System (5 to 50 ml/min)
HF Cleavage apparatus for Boc-chemistry
• AAPPTec Focus XC-4RV for Boc- and Fmoc-chemistry
• CEM Liberty Microwave for Fmoc-chemistry
Analytical and Preparative HPLC Systems
• Agilent 1100 and 1200 HPLC analytical systems
• Agilent 1100 LC/Electrospray MS system
• Beckman System Gold preparative HPLC system (10 ml/min max.)
• Sharp Semi-prep Gradient Purification System (5 to 50 ml/min)
HF Cleavage apparatus for Boc-chemistry
Peptide Synthesis (Solid-phase / solution phase)
Peptides can be synthesized using L- and/or D-amino acids or unusual amino acids. Boc-, Fmoc- or a combination of these synthesis chemistries can be used.
Peptide Purification (Reversed-phase HPLC)
Our facility only provides synthetic peptides at purities greater than 95% at the milligram to gram scale. All peptides are analyzed for purity by analytical reversed-phase HPLC and characterized by LC-MS (electrospray mass spectrometry).
Peptide Characterization (LC/MS)
Peptide Modification
See the rates and policies tab for possible modifications. If you don't see the modificiation you want, feel free to inquire.
Peptide Conjugation (polyclonal/monoclonal antibody production)
We can couple synthetic peptides to carrier proteins for production of polyclonal or monoclonal antibodies. We generally couple to Keyhole limpet hemocyanin (KLH) for antibody production and Bovine serum albumin (BSA) for screening antibodies by ELISA.
Quantitation (amino acid analysis)
Peptides can be synthesized using L- and/or D-amino acids or unusual amino acids. Boc-, Fmoc- or a combination of these synthesis chemistries can be used.
Peptide Purification (Reversed-phase HPLC)
Our facility only provides synthetic peptides at purities greater than 95% at the milligram to gram scale. All peptides are analyzed for purity by analytical reversed-phase HPLC and characterized by LC-MS (electrospray mass spectrometry).
Peptide Characterization (LC/MS)
Peptide Modification
See the rates and policies tab for possible modifications. If you don't see the modificiation you want, feel free to inquire.
Peptide Conjugation (polyclonal/monoclonal antibody production)
We can couple synthetic peptides to carrier proteins for production of polyclonal or monoclonal antibodies. We generally couple to Keyhole limpet hemocyanin (KLH) for antibody production and Bovine serum albumin (BSA) for screening antibodies by ELISA.
Quantitation (amino acid analysis)
PEPTIDE SYNTHESIS
Our staff has over 100 years of experience in peptide design and synthesis, and we are happy to discuss your order with you at no charge. Customers will receive a quotation prior to beginning production to assess costs. The costs of synthesis are dependent on peptide length and the incorporated modifications. Some bulk discounts may be available depending on the project.
Minimum charge for synthesis ($250)
0.1 mM scale: $25 per residue
0.25 mM scale: $40 per residue
All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and LC-MS
Preparative HPLC purification of synthetic peptides: $250 per peptide (depending on scale of synthesis)
Additional modification of peptides:
Incorporation of phosphorylated Ser or Thr
Incorporation of D-amino acids or other unusual amino acids
Acetylation
Biotin (C-terminal or N-terminal)
Fluorescent label
Conjugation to KLH or BSA
Methylation
AMINO ACID ANALYSIS
$40 per sample
LC/MASS SPECTROMETRY
LC-Mass determination of proteins, peptides, etc: $15 per sample
Contact Information
Peptide and Protein Chemistry FacilityUniversity of Colorado Anschutz Medical Campus
Bldg. RC-1 South, Room L18-1308
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045
Dr. Robert S. Hodges, Director
Phone: 303-903-5591
Fax: 303-724-3249
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L9121
Robert.Hodges@ucdenver.edu
Dr. Lajos Gera, Research Associate Professor
Phone: 303-724-3336
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308
Lajos.Gera@ucdenver.edu
Timothy Davis, Professional Research Assistant
Phone: 303-724-3336
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308
Timothy.Davis@ucdenver.edu